甜樱桃乙烯转录因子PaEIL1基因的克隆及序列分析

Cloning and Sequence Analysis of PaEIL1 Gene Encoding Ethylene Transcription Factors in Sweet Cherry Fruit

根据植物乙烯转录因子EIL(EIN3-like)家族的氨基酸和核苷酸保守区序列设计简并引物,以甜樱桃(Prunus aviumL.)果实的cDNA为模板,通过RT-PCR方法得到了PaEIL1部分序列。随后进一步通过RACE技术克隆得到了PaEIL1全长片段,其大小为2 636 bp,包含一个1 806 bp大小的完整开放阅读框(ORF)及部分非编码区序列,编码蛋白大小约为601个氨基酸。聚类分析结果表明,PaEIL1属于EIN3/EIL家族乙烯转录因子成员。

The fragment of PaEIL1 was isolated from mature sweet cherry fruit by RT-PCR using degenerate primers corresponding to the EIN3/EIL conserved region sequence.Both 5′ and 3′ end sequences of the cDNA were cloned using RACE technique.PaEIL1 was 2 636 bp in length,while the predicted open reading frame（ORF） of the cDNA was 1 806 bp and the deduced amino acid sequence of PaEIL1 consisted of 601 amino acids.The results of phylogenic analysis indicated that PaEIL1 could be classified into EIN3/EIL protein family.