扇贝多肽的毒理学研究

TOXICOLOGY STUDY ON POLYPEPTIDE FROM CHLAMYS FARRERI

目的研究扇贝多肽的毒理学作用,评价其安全性。方法以小鼠为受试动物,采用一次性静脉注射和腹腔注射给药两种方法,观察扇贝多肽的急性毒性反应;应用小鼠骨髓细胞微核试验、染色体畸变实验和伤寒沙门菌基因回复突变实验研究扇贝多肽的致突变作用。以大鼠为受试动物,通过血液生化学指标的检测和心、肝、脾、肺、肾等主要脏器的病理组织学检查研究扇贝多肽的长期毒性。结果扇贝多肽一次性静脉注射给药对小鼠产生的急性毒性反应的LD50为3 187.001 mg/kg,一次性腹腔注射给药未见明显毒性反应。扇贝多肽10 g/kg腹腔注射在小鼠骨髓细胞微核试验、染色体畸变实验及鼠伤寒沙门菌基因回复突变实验中未见致突变作用。长期毒性实验结果表明,扇贝多肽大剂量组(600 mg/kg)、中剂量组(300 mg/kg)和小剂量组(150 mg/kg)大鼠的血液生化指标均正常,主要脏器均未见明显病理形态学改变。结论扇贝多肽在本实验剂量范围内无明显毒副作用。

Objective To study the toxicology of polypeptide from Chlamys farreri（PCF） and evaluate its safety.Methods The mice were assigned to either intravenous or intraperitoneal PCF,the acute toxic reactions（ATR） of PCF were observed.The mutagenic action of PCF was investigated through the Ames test,micronucleus test and chromosomal aberration test;rats were used to investigate long-term toxicity of PCF through biochemical indexes of blood and histopathologic examination of heart,liver spleen,lung,and kidney.Results The LD50 of ATR in mice received single intravenous PCF was 3 187.001 mg/kg,no obvious toxic reaction was noted in mice with intraperitoneal injection.The Ames test,micronucleus test,and chromosomal aberration test showed that no mutagenic action was seen in mice with intraperitoneal PCF of 10 g/kg.A long-term toxicity test indicated that the blood biochemical indexes were all normal after injection of high,moderate and low dose（600,300 and 150 mg/kg）of PCF,and without manifestly pathomorphologic changes in main organs.Conclusion Within the dosage range used in the present study,PCF shows no obvious toxic or side effects.