新鲜自体心包作为瓣膜替代材料植入腹主动脉的研究

STUDY OF FRESH AUTOLOGOUS PERICADIAL VALVE IN ABDOMINAL AORTA AS HEART VALVE SUBSTITUE MATERIAL

目的观察新鲜自体心包作为瓣膜替代材料在腹主动脉内的转归。方法将新鲜自体心包瓣膜以及经2 g/L的戊二醛处理后自体心包瓣膜植入动物腹主动脉内,于术后3(T1)、6个月(T2)处死动物,取植入的组织,通过测量及组织学、透射和扫描电镜等方法观察自体心包瓣膜植入腹主动脉后,其延展性、组织相容性、钙化等方面的情况。结果各组心包瓣膜表面覆有单层完整的血管内皮细胞,瓣膜内有血栓形成。新鲜自体心包与自体腹主动脉组织连接紧密,其长度较植入时明显增加(F=223.44,q=3.73、3.86,P<0.05),且组织钙含量的增加程度较戊二醛处理后的心包瓣膜低(t=14.969、19.204,P<0.05)。结论新鲜自体心包作为心脏瓣膜替代材料具有一定的优势,尤其对于儿童瓣膜病的外科治疗具有一定意义。

Objective To study the fresh autologous pericardial valve in abdominal aorta as heart valve substitute.Methods Fresh and 2 g/L glutaraldehyde-treated autologous pericardial valve（each group,n=8） was implanted into swine abdominal aorta.When the pericardial valves were harvested at 3（T1） and 6（T2） months after the surgery（each time in each group,n=4）,with macroscopic and histological inspection,as well as observation under electron microscope,we studied the valves＇ expansibility,biocompatibility and calcification.Results There is a single layer of endothelial-like cell on the surface of all the pericardial valves and thrombus inside the valves.The connection between fresh autologous valves and aortic tissue is tighter,with positive expansion in their length than when implanted（F=223.44;q=3.73,3.86;P〈0.05）.Moreover,the increase of calcification in fresh valves is lower than treated ones（t=14.969,19.204;P〈0.05）.Conclusion Fresh autologous pericardial valves have great potential as heart valve substitute,and will be especially useful in surgical treatment for heart valve disease in children.