小鼠骨髓源树突状细胞体外诱导培养及初步鉴定

Culture and identification of mouse bone marrow-derived dendritic cells in vitro

用重组小鼠粒细胞-巨噬细胞集落刺激因子(rmGM-CSF)和重组小鼠白细胞介素4(rmIL-4)体外诱导小鼠骨髓细胞分化为树突状细胞,进行形态学变化观察,分析细胞表面分子,刺激T细胞增殖,探讨小鼠骨髓源树突状细胞(BMDC)体外诱导培养并进行初步鉴定。体外培养9d后BMDC可达80%以上,光镜下可见典型的树突状细胞形态。清楚表达成熟期主要表面标志物,可显著刺激同种异体混合淋巴细胞增殖。获得了较高纯度的BMDC,避免了使用传统磁珠分离方法所带来的成本高,操作复杂,产出率低的弊端,为研究BMDC功能以及运用开展下游实验提供材料。

To establish a method of cultivation and purification of dendritic cells（DC） from mouse bone marrow in vitro and observe their morphology,recombinant mouse granulocyte and macrophage colony stimulus factor（GM-CSF） and interleukin-4（IL4） induction were used to induce mouse bone marrow cells to form dendritic cells in vitro.After 9 days,the percentage of the cultured DCs was more than 80% with typical morphology of DCs under light microscope.The mature cells had clear marker on their surface.This meant that these used substances could stimulate allogenic mixed lymphocyte proliferation.