南蛇藤提取物对Hepa1-6荷瘤小鼠肝癌移植瘤及血管生成的影响

Effect of Nansheteng Extract on Transplanted Hepatic Carcinoma and Angiogenesis in Hepa1-6 Tumor Bearing Mice

目的观察南蛇藤乙酸乙酯提取物对Hepa1-6细胞荷瘤小鼠肝癌移植瘤及血管生成的影响。方法将C57BL/6小鼠随机分为正常组、模型组、顺铂组及南蛇藤高、中、低剂量组,每组10只。除正常组外,其他各组于左前肢腋部皮下接种小鼠肝癌细胞株Hepa1-6(1×106)0.1ml/只。接种后2周,顺铂组腹腔注射顺铂1mg/kg,南蛇藤低、中、高剂量组分别灌胃,药液浓度为10、20、40mg/kg,连续给药28d,末次给药24h后,检测肿瘤重量、移植瘤组织内肿瘤细胞的凋亡,检测移植瘤组织内血管内皮生长因子(VEGF)的表达。结果与模型组相比,南蛇藤提取物高、中、低剂量组平均瘤重均有所减少(P<0.05或P<0.01);南蛇藤中、高剂量组移植瘤内凋亡的肿瘤细胞较模型组明显增多,同时VEGF的表达较模型组明显减少。结论一定浓度的南蛇藤乙酸乙酯提取物有抑制肿瘤生长的作用,其机制可能与促进肿瘤细胞凋亡及抑制肿瘤血管生成有关。

Objective To investigate the inhibitive effect and mechanism of Nansheteng（Celastrus orbiculatus） extract on transplanted hepatic carcinoma and angio genesis in the Hepa1-6 tumor bearing mice.Methods Sixty C57BL/6 mice were randomized into normal control group,model group,cisplatin group,and Nansheteng extract large,middle,and small dosage groups,10 mice in each group.Except the normal control group,Hepa1-6 cells（1×106） 0.1ml were inoculated subcutaneously in the axilla of left anterior limb of each mouse.Two weeks later,cisplatin group was given cisplatin 1mg/kg by intraperitoneal injection,while Nansheteng large,middle,small dossage group was given Nansheteng extract of 10,20,40mg/kg respectively by gastric perfusion.All the mice were medicated for 28 days in succession and were sacrificed 24 hours after the last medication.The weights of tumors and apoptosis in the transplanted tumor tissues were measured. The expression of vascular endothelial growth factor（VEGF） in the transplanted tumor tissues was determined.Results Compared with the model group,the average weight of tumors in the Nansheteng extract large,middle and low dosage groups was reduced（P0.05 or P0.01）.The apoptosis in the transplanted tumor tissues of the medicated groups was significantly more than that of the model group,and at the same time the VEGF expression was significantly less.Conclusion Nansheteng ethyl acetate extract is effective in inhibiting the tumor growth.Its mechanism may be related to promoting the apoptosis and inhibiting the angiogenesis of tumor.