不同植物对五氯酚生物富集能力的研究

Study on Bioaccumulation of Pentachlorophenol in Different Kinds of Plants

在添加2 000μg/kg五氯酚的土壤中种植芦苇(Phragmites communis(L.)Trin)、香蒲(Typha orientalis Presl)和水葱(Scirpus tabernaemontani Gmel),探讨不同植物对土壤中五氯酚的富集作用。植物和土壤样品以石油醚振荡提取90 min,浓硫酸净化和碳酸钠反萃取后,在pH为9的条件下采用衍生萃取同步进行处理样品20 min,以GC-μECD分离测定。通过30天的模拟野外生态试验,发现不同植物对五氯酚富集能力由大到小的顺序依次为水葱、香蒲、芦苇,研究显示植物修复对于土壤中持久性有机污染物五氯酚是一项切实可行的技术。

A method for determining residues of the persistent organic pollutants（POPS） pentachlorophenol（PCP） in environment was founded by means of GC-μECD.Samples were extracted with petroleum ether about 90 min by means of vibrating,and re-extracted with sodium carbonate,derivated with acetic anhydride about 20 min under the condition of pH 9 and at the same time extracted,finally analyzed by GC-μECD.The use of plants to detoxify contaminated soil was a potentially cost-effective alternative to traditional remediation technologies.The different plants ability of bioaccumulation and biodegradation of PCP from soil were investigated.The initial concentration of PCP was 2 000 μg/kg,at the end of the experiment after 30 days,chinese shallot（Scirpus tabernaemontani Gmel） absorbed most PCP,cattail（Typha orientalis Presl） absorbed more and reed（Phragmites communis（L.） Trin） absorbed lest from the soil.Generally speaking,the determination method was simple,sensitive,stable,quick and can be used as a routine method for determination of trace PCP in environment and it was a feasible technology to utilizing plants to enhance the degradation of organic contaminants such as PCP in soil systems.