摘要
[目的]研究野生型拟南芥叶片愈伤组织的诱导。[方法]以野生型拟南芥叶片为外植体,通过将其切段后接种在添加有不同浓度6-BA和NAA的MS培养基上,研究了拟南芥愈伤组织的诱导和再生植株。[结果]6-BA对于愈伤组织诱导不可缺少,但其浓度太高易产生玻璃化;NAA单独使用利于生根,配合6-BA使用利于分化芽;获得最适愈伤组织诱导培养基为MS+0.50mg/L6-BA+0.10mg/LNAA,愈伤组织诱导率可达100%。[结论]为进一步开展拟南芥的遗传转化或细胞培养奠定了基础。
[Objective] The callus induction of wild Arabidopsis thaliana leaves was studied.[Method] As explants,leaf segments of wild A.thaliana were inoculated in MS medium including 6-BA and NAA with different concentration,and studying the callus induction and regenerated plant of A.thaliana.[Result]6-BA was necessary for callus induction,but higher concentration resulted into vitrification easily;single use of NAA was beneficial to rooting,and bud differentiation was easy in the medium with NAA and 6-BA;the optimum medium of callus induction was MS ＋0.5 mg/L 6-BA ＋0.10 mg/L NAA,with the callus induction frequency of 100%.[Conclusion]Our study could lay a foundation for the genetic transformation and cell culture of A.thaliana.
基金
Supported by Basic Scientific Research Special Found of Central College(100030-2120131106)~~
关键词
拟南芥
叶片
愈伤组织
Arabidopsis thaliana
Leaf
Callus
