Optimization of Callus Induction Medium for Schisandga chinensis Baill via Uniform Design

均匀设计法优化北五味子愈伤组织诱导培养基(英文)

[Objective]This study was to optimize callus induction medium for Schisandga chinensis Baill.[Method]Using callus induction rate as an indicator,uniform design was employed to optimize hormone combination at poly-factors and poly-levels for callus induction from Schisandga chinensis Baill.[Results]Optimal hormone combinations depended on different explants：optimum medium for both tender leaf and petiole was MS ＋3 mg/L 6-BA,for stem segment was MS ＋3 mg/L 6-BA ＋0.6 mg/L NAA.[Conclusion]Uniform design is a time-saving and convenient method for the optimum medium for callus and yields a higher callus induction rate.

[目的]优化北五味子愈伤组织诱导培养基。[方法]采用均匀设计法,以诱导率为主要考察目标,对北五味子愈伤组织生长的激素成分进行多因素多水平考察。[结果]叶片的最佳培养基组合为MS+3mg/L6-BA;叶柄的最佳培养基组合为MS+3mg/L6-BA+0mg/LNAA;茎段的最佳培养基组合为MS+3mg/L6-BA+0.6mg/LNAA。[结论]用均匀设计法优化愈伤组织诱导培养基省时、方便,且明显高于均匀设计实验方案中的诱导率。