丙烯腈对大鼠肝脏Ca^(2+)-ATP酶、磷酸化酶A活力的影响

Effect of acrylonitrile on the activity of Ca 2+ ATPase and phosphorylase A of liver in rats

本文通过丙烯腈对大鼠肝脏Ｃａ２＋－ＡＴＰａｓｅ和磷酸化酶Ａ活性影响的研究，探讨了丙烯腈对动物肝脏钙稳态的影响，进一步阐明丙烯腈的毒作用机制。采用成年雄性ＳＤ大鼠，每天经口染毒，染毒剂量为０、１０、３０和５０ｍｇ／ｋｇ，连续染毒４２天，于染毒第１４天、２８天和４２天时分别测定各组肝匀浆Ｃａ２＋－ＡＴＰａｓｅ和磷酸化酶Ａ的活性。结果显示，丙烯腈可明显地抑制肝脏Ｃａ２＋－ＡＴＰａｓｅ（Ｐ＜０．０１），同时可显著地激活磷酸化酶Ａ的活性，特别是高剂量组（５０ｍｇ／ｋｇ）和染毒４２天时影响最明显，相关关系分析表明，丙烯腈对Ｃａ２＋－ＡＴＰａｓｅ和磷酸化酶Ａ的活性的影响均存在较好的剂量－反应和时间－反应关系（Ｐ＜０．０１）。结果认为丙烯腈能显著地抑制大鼠肝脏Ｃａ２＋－ＡＴＰａｓｅ和激活磷酸化酶Ａ的活性，可导致大鼠肝脏钙稳态的失调。

The activity of Ca 2+ ATPase and phosphorylase A(P A) of liver in rats was determined to study the effect of acrylonitrile (ACN) on calcium homeostasis and to clarify the toxicological mechanism of ACN. Adult male Sprague Dawley rats were administered ACN daily per os for 42 days, at the dosage of 0,10,30 and 50 mg·kg -1 . The activities of Ca 2+ ATPase and phosphorylase A was determined at 14,28,42 days after ACN administration. The results indicated that ACN could significantly inhibit the activity of Ca 2+ ATPase and increase the activity of P A ( P <0 01),especially in the high dose group and exposed for 42 days, and there was significantly dose and time respond relationship ( P <0 01). ACN contamination could result in the disharmony of calcium homeostasis of liver in rats.