微波对视网膜神经节细胞的过氧化损伤

Lipid peroxide damage in retinal ganglion cells induced by microwave

目的：探讨微波对体外培养的猪视网膜神经节细胞的脂质过氧化损伤作用及耐受剂量，可为进一步研究微波的眼底损伤机理及其防护提供一定的实验依据。方法：体外培养猪视网膜神经节细胞，２４５０ＭＨｚ连续波以不同强度和时间进行辐照，然后继续培养４８小时，测定超氧化物歧化酶（ＳＯＤ）和丙二醛（ＭＤＡ）含量。结果：微波辐照后，细胞ＭＤＡ含量明显增高，ＳＯＤ降低，经过４８小时再培养，ＭＤＡ含量有所恢复，ＳＯＤ含量增高。结论是：微波可引起视网膜神经节细胞脂质过氧化损伤，在一定剂量范围内为可逆性损伤。

The determination of lipid peroxide damage in the primary cultured pig retinal ganglion cells induced by microwave can provide some experience on the effect of microwave and the protection from its damage. Retinal ganglion cells were cultured in vitro and exposed to different intensities or time of microwave,and cultured for another 48 hours.The content of superoxide dismutase (SOD) and malondialdehyde (MDA) was detected.Results showed that the content of MDA was increased and SOD decreased after radiation.After 48 hours in culture,the content of MDA recovered, and SOD increased.It is concluded that microwave induced the lipid peroxide damage in primary cultured retinal ganglion cells.These damages were recoverable in certain range of indensity or time.