食品中分离的李斯特氏菌的致病基因分析

Pathogenic gene analysis of Listeria isolated from foods

１９９６—１９９７年作者对全国１２个省市的７类食品共３７４６份进行了李斯特氏菌的污染调查，共分离出李斯特氏菌１６５株。用ＰＣＲ技术对分离菌株中李斯特氏菌的２种主要致病因子李氏溶血素Ｏ和内化素的基因进行了检测，其中５７株仅有内化素基因，２７株同时具有内化素基因和李氏溶血素Ｏ基因。两种致病基因均未检出的共有８１株。本研究说明判断单增李氏菌的主要指标是小鼠毒力试验阳性而不是其溶血反应，同时检测溶血素Ｏ和内化素２种基因对单增李氏菌有鉴别意义。

In 1996～1997, Listeria in foods from 12 provinces of China were detected by using the national standard examination method GB 4789.30-94(routine traditional methods).Twenty four strains Listeria monocytogenes and 141 strains Listeria were identified by biochemical procedures and animal toxicity experiment.Polymerase Chain Reaction(PCR) was used to amplify two specific DNA fragments(719bp and 446bp) of listeriolysin O and internalin genes with two pares of primer Hly 1-2 and Inl 1-2 ,respectively.The experiment results showed that 57 strains contained only internalin,27 strains contained both internalin and listeriolysin O from the pathogenic genes of isolated strains.