不同饲料构成对大鼠肥胖基因表达产物——瘦素的影响

Effect of Fodder Components on The Product of Obese Gene Expression——Leptin in Rats

目的 研究不同饲料构成和不同能量摄入水平对大鼠肥胖基因表达产物———瘦素、体内胰岛素、生长激素分泌的影响及它们之间存在的相互关系。方法 采用 Ｗｅｓｔｅｒｎ ｂｌｏｔ 检测血浆瘦素，采用放射免疫学方法检测血清中胰岛素、生长激素。结果 在能量摄入水平相同情况下，不同饲料构成包括高蛋白、高脂肪、高碳水化合物对瘦素、胰岛素和生长激素分泌没有明显的影响；而能量摄入水平对上述３ 个指标均有明显的影响，即在能量摄入水平增高的情况下，ｏｂ 基因表达水平增高，表现为血浆瘦素水平升高，同时胰岛素分泌增加，生长激素分泌减少。结论 瘦素、胰岛素和生长激素的分泌三者之间可能存在相互作用机制，并受共同代谢信号调节。

Objective To study the effects of different fodder components and levels of caloric uptake on the product of obese gene expression-leptin and in vivo secretion of insulin and growth hormone, and to study the relationship between them in rats. Methods Plasma leptin was determined by Western blot, and serum insulin and growth hormone were determined by radioimmunoassay (RIA). Results There was no significant effects of fodder components, including high protein, fat and carbohydrate, on the levels of leptin, insulin and growth hormone in rats, with the same caloric uptake in them. However, with the higher caloric uptake, level of obese gene expression increased, showing increased levels of plasma leptin and serum insulin and decreased level of serum growth hormone. Conclusion There could existed an interaction between expression of obese gene and secretions of leptin, insulin and growth hormone, which could be modified by the same metabolic signal.