烟溶液与温石棉对细胞增殖活性的影响

Effects of Cigarette Smoke Solution and Chrysotile on Proliferation Activity of Human Embryo Lung Cells

目的 探讨香烟烟雾溶液与温石棉单独及联合作用对细胞增殖活性的影响。方法 将人胚肺（ Ｈ Ｅ Ｌ） 成纤维细胞用不同浓度的烟溶液和温石棉单独及联合作用一定时间后，应用［３ Ｈ］ Ｔｄ Ｒ参入法测定了细胞的增殖能力。结果 低剂量的烟溶液与温石棉单独作用时均可促进细胞的增殖，但较高剂量的烟溶液和温石棉则可抑制细胞的增殖；当二者联合作用时，低剂量的烟溶液与温石棉可协同增加细胞的增殖，而较高剂量的烟溶液与温石棉则只表现为相加作用。结论 烟溶液与温石棉单独及联合作用均可影响 Ｈ Ｅ Ｌ细胞的增殖活性，但其促增殖和抑制增殖能力则随剂量不同而不同。

Objective To study the separate and combined effects of cigarette smoke solution and chrysotile on cell proliferation activity.Methods Human embryo lung (HEL)fibroblasts were cultivated with cigarette smoke solution and chrysotile separately or in combination at varied concentrations for certain times,and cell proliferation was observed with [ 3H]TdR incorporation.Results Cigarette smoke solution and chrysotile could enhance cell proliferation at lower doses,both separately or in combination,but inhibit it at higher doses. Lower dose of cigarette smoke solution and chrysotile in combination could enhance cell proliferation synergistically,but higher doses could only do additively.Conclusion Cigarette smoke solution and chrysotile could affect HEL cell proliferation separately or in combination,with an enhancing or inhibiting effect,which depended on their doses.