碱性成纤维细胞生长因子的放射免疫分析

Radioimmunoassay of basic fibroblast growth factor

目的：建立及评价碱性成纤维细胞生长因子（ｂ Ｆ Ｇ Ｆ）的１２５Ⅰ标记及放射免疫分析（ Ｒ Ｉ Ａ）方法。方法：采用氯胺 Ｔ 氧化法进行 ｂ Ｆ Ｇ Ｆ １２５Ⅰ标记，并以双抗体 Ｐ Ｅ Ｇ 的 Ｒ Ｉ Ａ 测定家兔血清 ｂ Ｆ Ｇ Ｆ水平。结果：１２５Ⅰ标记ｂ Ｆ Ｇ Ｆ的碘利用率为８１９８％ ，比放射性为３０３ Ｍ Ｂｑ／μｇ，放化纯度达 ９５０％以上；ｂ Ｆ Ｇ Ｆ 放免分析灵敏度可达 ０４ ｎｇ。结论：本研究为 ｂ Ｆ Ｇ Ｆ 的１２５Ⅰ标记及定量测定提供了简便可靠的方法。

Objective:The present study was to establish and estimate a 125 Ⅰ label and an radioimmunoassay (RIA) of basic fibroblast growth factor (bFGF).Methods:The iodinated recombinant human bFGF (rhbFGF) was prepared using chloramine T (Ch T),and levels of bFGF in rabbit serum were determind by a double antibody polyethylene glycol (PEG) RIA protocol.Results:The radioiodine utilization ratio,specific radioactivity and radiochemical purity of 125 Ⅰ labeling bFGF were 81.98%,3.03 MBq/μg and over 95%,respectively.The sensitivity of bFGF RIA reached 0.4 ng.Conclusion:The present study may provide a convenient and reliable method for 125 Ⅰ label and quantitative determination of bFGF.