脑室注入 LaCl_3对 ARCN、 DMN 和 VMN 核电生理活动的影响

Effect of LaCl 3 on electrophysiologic activity of ARCN,DMN and VMN

目的：观察侧脑室注入氯化镧（ Ｌａ Ｃｌ３）对大鼠下丘脑弓状核（ Ａ Ｒ Ｃ Ｎ）、腹内侧核（ Ｖ Ｍ Ｎ）、背内侧核（ Ｄ Ｍ Ｎ）神经元电活动的影响。方法：侧脑室注入不同浓度的 Ｌａ Ｃｌ３，用细胞外放电记录技术，观察注药后上述核团放电频率的变化。结果：小剂量 Ｌａ Ｃｌ３ （００１ μｍ ｏｌ）注入上述核团后，神经元放电频率明显增加，而增大 Ｌａ Ｃｌ３注射浓度后，神经元放电频率多表现为明显减少，且有剂量依赖趋势，１０ μｍ ｏｌ的 Ｌａ Ｃｌ３比０１ μｍ ｏｌ抑制作用更明显。结论：大鼠下丘脑 Ｄ Ｍ Ｎ， Ｖ Ｍ Ｎ 和 Ａ Ｒ Ｃ Ｎ 中神经元对脑室注射 Ｌａ Ｃｌ３的反应是小剂量呈兴奋作用，而大剂量则为抑制效应。

Objective:To observe the effect of LaCl 3 on electrophysiologic activity of arcuatus nucleus (ARCN),dorsomedial nucleus (DMN) and ventromedial nucleus (VMN) after injection into lateral ventricle of rats.Methods:Frequency of extrocellular discharges of nuclei before and after injection LaCl 3 into lateral ventricle was recorded with electrophysiologic technique.Results:Small dose of LaCl 3 (0.01 μmol) induced the increase of extrocellular discharge.Large dose of LaCl 3 made the discharge decrease.The inhibited effect of 1.0 μmol was more powerful than that of the 0.1 μmol.Conclusion:The effect of LaCl 3 on nuclei in hypothalamus of rat is dose dependent:small dose produced exciting reaction and large dose showed inhibited role.