小儿肺炎与一氧化氮、肿瘤坏死因子的相关性研究

Changes in serum nitric oxide and tumor necrosis factor in children with pneumonia

目的：阐明肺炎时一氧化氮（ Ｎ Ｏ）和肿瘤坏死因子（ Ｔ Ｎ Ｆ）的变化。方法：采用比色法、双抗体夹心 Ｅ Ｌ Ｉ Ｓ Ａ 法分别测定４１例肺炎患儿及２５例正常健康儿血清 Ｎ Ｏ、 Ｔ Ｎ Ｆ水平；并研究了 Ｎ Ｏ 与 Ｔ Ｎ Ｆ的关系。结果：急性期肺炎患儿的 Ｎ Ｏ、 Ｔ Ｎ Ｆ 较对照组、恢复期患儿明显升高；重症组较轻症组明显升高；而且 Ｎ Ｏ 与 Ｔ Ｎ Ｆ呈高度正相关。结论：血清 Ｎ Ｏ、 Ｔ Ｎ Ｆ 水平与肺炎的发生、发展和病情严重程度有关。

Objective:The study was to explain some mechanism of pneumonia and clinical significance of NO and TNF.Methods:The authors measured the levels of NO and TNF in 41 patients with pneumonia and 25 healthy controls by using colorimety and two step sandwich ELISA technique,respectively.Results:The results showed that the levels of NO and TNF in pneumonia children were higher than those in the controls and recovery group;the levels of NO and TNF in severs cases were higher than those in patients with mild symptoms.Moreover,Serum NO level positively correlated with TNF level.Conclusion:The results of the study suggest that NO and TNF are associated with the develpment,progress and severity of pneumonia in children.