点突变对ColE2质粒复制起始部位生物活性的影响

Mutations at Position 13 Lead to a Decrease of the Biological Activities of ColE2 Replication Origin

为了研究ＣｏｌＥ２质粒复制起始部位中的一些位点和其生物活性的关系，用人工合成寡核苷酸定点引入点突变的方法，对ＣｏｌＥ２质粒复制起始部位的１个位点引入３种点突变，并测定了突变的ＣｏｌＥ２质粒复制起始部位的Ｒｅｐ蛋白结合和复制活性。结果表明，该位点的突变导致ＣｏｌＥ２质粒复制起始部位的生物活性明显下降。说明该位点对ＣｏｌＥ２质粒复制起始部位和Ｒｅｐ蛋白的结合及复制起始都起一定作用。

In order to study the structure and function of the replication origin in plasmid ColE2,mutations were introduced into the position 13 in ColE2 origin.The assays of the in vivo Rep proteinbinding and replication activities of the mutant ColE2 origins show that the mutation of this position leads to a remarkable decrease of the biological activities of ColE2 origin.It is concluded that this position is important to the functions of ColE2 origin.