摘要
在被扩增的DNA片段存在特殊结构时,通过改变PCR反应成分,并采用不同的退火温度加热启动,得到了满意的特异的PCR扩增产物。
The specific PCR product and improved yield for problematic template caused by GCrich region were obteined by modifying the components in PCR reaction using of hot start and alternative annealing temperature.
出处
《苏州医学院学报》
1999年第7期788-789,共2页
Acta Academiae Medicinae Suzhou
关键词
DNA
二级结构
聚合酶链反应
反应条件
DNA secondary structure
polymerase chain reaction
reaction conditions