糖尿病大鼠血清、组织一氧化氮含量的测定及其意义

DETERMINATION OF NITRIC OXIDE IN SERUM AND TISSUE OF EXPERIMENTAL DIABETIC RATS

目的：探讨一氧化氮（NO）与糖尿病（DM）及其并发症发病的相关性。方法：通过检测链脲佐菌素（STZ）所致糖尿病（DM）大鼠血清及心肌、肾脏组织匀浆中NO的代谢产物亚硝酸盐及硝酸盐（NO2-NO3-）的含量以反映机体内源性NO水平，同时检测其它相关的生化指标。结果：DM大鼠血清、心、肾组织匀浆中NO2－NQ3－含量显著升高（P＜0．001），同时伴随血清尿素氮（BUN）、肌酐（CRE）、血清谷草转氨酶（SGOT）、肌酸磷酸激酶－MB（CK－MB）等生化指标及血清、组织总抗氧化能力的改变。结论：DM大鼠存在内源性NO生成亢进，可能参与DM及其并发症的发生、发展。

Objective: To discuss the relationship between nitric oxide (NO)and diabetic mellitus (DM) and its chronic complications. Methods: The model of DM was built by streptozotocin (STZ). The concentration of nitrate (NO3-) and nitrite (NO2-) which reflects the level of endogenous nitric oxide was measured in serum and tissue of experimental diabetic rats. Results: The levels of NO2-NO3-in serum heart and kidney of experimented diabetic rats incrased markedly(p <0.001). In the meantime, the levels of BUN, CRE, SGOT and CK-MB in serum increased, but the total ability of anti-oxidation decresed significantly(P<0.01). Conclusions: It is suggested that DM is associated with high level of endogenous NO, which may participate in the happening and development of DM and its chronic complications.