甲拌磷降解菌XT2的分离鉴定及其降解特性

Isolation and Characteristics of a Phorate Degradation Bacterium XT2

[目的]选用新疆石河子市周边受甲拌磷污染的土壤为菌源进行研究,从而获得有效降解有机磷农药甲拌磷的微生物。[方法]以甲拌磷作为唯一碳源和能源采用逐渐加量的驯化方式。[结果]分离得到1株对甲拌磷有一定降解能力的降解菌XT2,根据其形态特征、生理生化特性及16S rDNA序列分析,将其鉴定为斯氏普罗威登斯菌(Providencia stuartii),该菌能以甲拌磷作为唯一碳源生长,接种该菌于500 mg/L的甲拌磷基础盐液体培养基中,120 h时甲拌磷降解率可达77.89%。[结论]筛选获得了1株能有效降解甲拌磷的细菌,被鉴定为斯氏普罗威登斯菌。

Soil from village near Shihezi,where phorate had been previously applied,was used as a source ofbacteria capable of degrading this organophosphorus pesticide.[Methods]By using phorate as the sole carbon and energysource for bacterial growth,the dose was gradually increased.[Results]The XT2 bacterial strains was isolated with someability to break down phorate.The bacteria was identified as Providencia stuartii based on physiological,biochemicalcharacteristics and 16S ribosomal DNA sequence analysis and XT2 could use phorate as the sole carbon source.The rateof degrading prorate was up to 77.89%after 120 hours in the condition of 500 mg/L phorate.[Conclusions]A bacterialstrains with ability to break down phorate was obtained and was identified as Providencia stuartii.