摘要
目的:建立人腮腺多形性腺瘤有限细胞系。方法:取腮腺多形性腺瘤患者原发肿瘤进行原代培养。观察细胞的形态特征,绘制细胞生长曲线,采用免疫细胞化学检测细胞内特异性抗原标志物,对细胞进行鉴定。结果:多形性腺瘤细胞体外培养时间约为80d,传代至16代,细胞呈短梭形、多边形,肿瘤性腺上皮细胞角蛋白弱阳性,肿瘤性肌上皮细胞肌动蛋白阳性、波形蛋白阳性。结论:多形性腺瘤细胞在体外培养连续传代,为永生化腮腺多形性腺瘤细胞系的建立奠定了基础。
PURPOSE： To establish the limited generation of cell line of human parotid pleomorphic adenoma（PA）.METHODS： The sample was obtained from a male patient with parotid pleomorphic adenoma.Growth curve was madefollowing the 7th passage cells.PA cell morphological characteristics were observed by phase-contrast invert microscopeand identified by means of immunohistochemistry.RESULTS： PA cells were maintained in culture for over 2 months.PAcells were short spindle-shaped or polygonal in vitro.The antibody against cytokeratin was labelled in the cytoplasms ofadenoepithelial cells,and myoepithelial cells were positive for the antibody against smooth muscle actin and vimentin.CONCLUSION： The culture of PA cells could continuously deliver passage in vitro,and can be used to prepared forestablishing immortalized pleomorphic adenoma cell lines.Supported by Research Fund of Science and TechnologyCommission of Shanghai Municipality（08JC1412900） and Shanghai Leading Academic Discipline Project（S30206）.
出处
《中国口腔颌面外科杂志》
CAS
2011年第2期108-111,共4页
China Journal of Oral and Maxillofacial Surgery
基金
上海市科学技术委员会重点项目(08JC1412900)
上海市重点学科建设项目(S30206)
关键词
多形性腺瘤
原代培养
细胞系
Pleomorphic adenoma
Primary culture
Cell lines
