闭合性脑损伤后血清和海马的蛋白质表达

Expression of Proteins in Serum and Hippocampus after Closed Brain Injury in Rats

目的研究大鼠脑损伤后血清和海马中蛋白质表达的差异。方法用雄性SD大鼠制造脑损伤模型,应用弱阳离子交换(WCX2)芯片和铜离子结合(IMAC-Cu)芯片结合表面增强激光解析电离飞行时间质谱技术,分析大鼠闭合性脑损伤后不同时间血清和海马中蛋白质表达谱的变化。结果 WCX2芯片在血清和海马样本中分别捕获436个和364个蛋白质峰,IMAC-Cu芯片在血清和海马样本中分别捕获229个和345个蛋白质峰。WCX2芯片在血清和海马中都能捕获的蛋白质峰为10个,IMAC-Cu芯片在血清和海马中都能捕获的蛋白质峰为13个。结论闭合性脑损伤可引起血清和海马蛋白质表达谱发生变化,且二者存在显著差异,同种芯片在血清和海马中检测到的差异蛋白质,可能是脑损伤生物标志蛋白。

Objective To explore the difference of expression of proteins between the serum and hippocampus after brain injury in rats.Methods Male SD rats were used to establish brain injury model.The changes of proteins expression profile in serum and hippocampus at different time after brain injury were analyzed using weak cationic exchanger（WCX2） chips and immobilized metal affinity capture arrays-Cu（IMAC-Cu） chips by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry.Results A total of 436 protein peaks were detected in serum and 346 protein peaks were detected in hippocampus using WCX2 chips.A total of 229 protein peaks were detected in serum and 345 protein peaks were detected in hippocampus using IMAC-Cu chips.The same 10 protein peaks were respectively detected in serum and hippocampus using WCX2 chips.The same 13 protein peaks were respectively detected in serum and hippocampus using IMAC-Cu chips.Conclusion The changes of protein expression profile in serum and hippocampus are obvious after closed brain injury and show a significant difference.The different proteins detected in serum and hippocampus using the same chip could be biochemical markers for determining brain injury.