犬组织中艾司唑仑在甲醛溶液中的稳定性

Stability of Estazolam in Dog Tissues Preserved in Formaldehyde Solution

目的研究甲醛溶液保存的生物样品中艾司唑仑的稳定性。方法犬以艾司唑仑37.6mg/kg剂量灌胃,2h处死,解剖取其心、肝、肾、脑并分割为1g/份,分别放置于4%甲醛溶液中固定,用高效液相色谱法测定各组织及甲醛液中的艾司唑仑含量。结果犬心、肝、肾、脑组织随着固定在甲醛溶液中时间的延长,组织及从组织溶解到甲醛溶液中的艾司唑仑含量均降低,保存至63d时,心、肝、肾和脑组织中艾司唑仑检出量分别为固定前的0.8%、1.7%、1.0%和2.2%。结论犬组织中的艾司唑仑会部分溶解到甲醛溶液中,艾司唑仑在甲醛溶液中容易分解。含艾司唑仑的生物样品应避免保存在甲醛溶液中。

Objective To investigate the stability of estazolam in biological samples preserved in formaldehyde solution.Methods The dog was given intragastric administration of estazolam with a dose of 37.6 mg/kg and killed 2 h later.Heart,liver,kidney and brain of the dog were cut up into 1 g and preserved in 4% formaldehyde solution respectively.The content of estazolam in biological samples and formaldehyde solution were analyzed by HPLC at different times.Results The content of estazolam in heart,liver,kidney and brain or in formaldehyde solution reduced gradually followed with the extention of preservation time.At the 63 th day,estazolam content in four tissues were 0.8%,1.7%,1.0% and 2.2% of the original content respectively.Conclusion Estazolam in tissues can diffuse into formaldehyde solution and decomposed quickly,so biological samples contained estazolam should not be preserved in formaldehyde solution.