小麦成熟胚离体培养研究

Study on Mature Wheat Embryos Culture in Vitro

以美国小麦品种Overley成熟胚作为外植体进行离体培养,研究外源激素对小麦成熟胚诱导愈伤组织、分化出苗以及试管苗增殖的影响。试验结果表明,在MS培养基中添加2,4-D3mg·L-1愈伤组织诱导频率较高,成愈率为84.0%;在添加有KT2～3mg·L-1的MS分化培养基中愈伤组织分化率较高,可达85%以上;将试管苗转接至添加0.2mg·L-1的NAA和0.5～2.0mg·L-1的6-BA的培养基中,试管苗有一定的分化增殖,但最高分化率仅为33.3%,增殖系数2.05。

Mature wheat embryos of Overley were used as explants for culture in vitro.The result showed that the optimum medium for callus induction was MS＋2,4-D（3.0 mg·L-1）,the rate of callus formation was 84.0%.The medium for callus differentiation was MS＋KT（2~3 mg·L-1）,the differentiation of callus was more than 85%.The wheat plantlets had low differentiation rate and multiplication coefficient in MS＋NAA（0.2 mg·L-1）＋6-BA（0.5~2.0 mg·L-1）,which were 33.3% and 2.05.