摘要
以美国小麦品种Overley成熟胚作为外植体进行离体培养,研究外源激素对小麦成熟胚诱导愈伤组织、分化出苗以及试管苗增殖的影响。试验结果表明,在MS培养基中添加2,4-D3mg·L-1愈伤组织诱导频率较高,成愈率为84.0%;在添加有KT2~3mg·L-1的MS分化培养基中愈伤组织分化率较高,可达85%以上;将试管苗转接至添加0.2mg·L-1的NAA和0.5~2.0mg·L-1的6-BA的培养基中,试管苗有一定的分化增殖,但最高分化率仅为33.3%,增殖系数2.05。
Mature wheat embryos of Overley were used as explants for culture in vitro.The result showed that the optimum medium for callus induction was MS+2,4-D(3.0 mg·L-1),the rate of callus formation was 84.0%.The medium for callus differentiation was MS+KT(2~3 mg·L-1),the differentiation of callus was more than 85%.The wheat plantlets had low differentiation rate and multiplication coefficient in MS+NAA(0.2 mg·L-1)+6-BA(0.5~2.0 mg·L-1),which were 33.3% and 2.05.
出处
《天津农业科学》
CAS
2011年第2期15-17,共3页
Tianjin Agricultural Sciences
基金
天津市科技成果转化及产业化推进计划项目(08ZHNZNC03400)
天津农学院科学研究发展基金计划项目
关键词
小麦
成熟胚
离体培养
植株再生
wheat
mature embryo
in vitro culture
plant regeneration