HPLC法同时测定苦黄软膏中大黄素、大黄酚的含量

Simultaneous determination of two contents of rhubarb in kuhuang ointment by HPLC

目的建立高效液相色谱法（HPLC）同时测定苦黄软膏中大黄素、大黄酚含量的方法。方法采用SymmetryC18柱（250mm×4.6 mm,5μm）,甲醇-0.1%磷酸（85：15）为流动相,流速1.0 ml·min-1,柱温25℃,检测波长254 nm。结果大黄素在0.320~1.60μg（r=0.999 5）,大黄酚在0.132~0.660μg（r=0.999 2）范围线性良好;平均回收率分别为98.02%、98.15%;RSD分别为1.66%、1.52%。结论该方法简便、可靠、准确,可作为苦黄软膏的质量控制方法。

Aim To establish an HPLC method for the determination of emodin and chrysophanol in kuhuang ointment.Methods The determination was performed on symmetry-C18 column（250 mm×4.6 mm,5 μm）with mobile phase consisted of methanol-0.1% phosphoric acid（85∶15）at a flow rate of 1.0 ml·min-1.Column temperature was 25℃.The UV detection wavelength was set at 254 nm.Result The calibration curves were linear in the range of 0.320～1.60 μg（r=0.999 5）for Emodin,0.132～0.660 μg（r=0.999 2）for chrysophanol.The average recovery was 98.02%,98.15% and RSD was 1.66%,1.52% respectively.Conclusion The determination method is simple,reliable,accurate,and it can be used for quality control of Kuhuang Ointment.