HPLC法测定不同产地博落回中白屈菜红碱和血根碱含量

Determination of chelerythrine and sanguinnarine in macleaya cordata from the different areas by HPLC

目的建立不同产地博落回根中白屈菜红碱和血根碱的HPLC法含量测定方法。方法采用HPLC法,色谱柱:SHI-MADZU C18(4.6 mm×250 mm,5μm),流动相:0.4%磷酸-乙腈(73:27),流速1.2 ml·min-1,柱温30℃。结果白屈菜红碱、血根碱的回收率分别为98.26%,97.73%;RSD分别为1.87%,1.81%。结论利用该法测定博落回总碱中的白屈菜红碱和血根碱,结果准确度高、精密度好,可作为博落回总碱的质量控制方法。

Aim To develop an HPLC method for the determination of chelerythrine and sanguinairne in macleaya cordata from different place of production.Methods HPLC was adopted,SHIMADZU C18 column（4.6 mm×250 mm,5 μm）with a mobile phase acetonitrile-0.4%（V/V）phosphoric acid solution（27∶73）by diode array detector at 270 nm.Results The average recoveries of chelerythrine and sanguinarinc were 98.26%,97.73%,respectively.RSD were 1.87%,1.81%respectively.Conclusion The method is sensitive and reliable and can be used as a quality control method of the chelerythrine and sanguinairne in total alkaloid of macleaya cordata.