雌激素受体α基因多态性与子宫内膜增生症的相关性研究

Relationship Between Estrogen Receptor alpha Gene Polymorphism and Endometrial Hyperplasia

目的探讨雌激素受体α(ERα)基因多态性与子宫内膜增生症的关系。方法选择江西地区100例子宫内膜增生症患者为实验组,100例子宫内膜正常者为对照组。应用分子生物学的方法分析ERα基因1号内含子内切酶XbaⅠ和PvuⅡ限制性片段长度多态性(restriction fragment length polymorphism,RFLP),观察ERα基因多态性基因型在2组中的基因型分布。RFLP用PP、Pp、pp(PvuⅡ)和XX、Xx、xx(XbaⅠ)来表示。结果 X基因型频率实验组为35.0%,对照组为24.5%(OR=1.659,P=0.022);P基因型频率实验组为23.0%,对照组为34.5%(OR=0.567,P=0.011);XbaⅠ和PvuⅡ限制性片度长度多态性在2组中均呈多态性分布。结论 ERα基因多态性与子宫内膜增生症有关,X等位基因可能是其危险因素;P等位基因可能是其保护因素。

Objective To investigate the relationship between estrogen receptor alpha（ERα） gene polymorphism and endometrial hyperplasia.Methods One hundred patients with endometrial hyperplasia（case group） and 100 women with normal endometria（control group） were recruited in Jiangxi province.Restriction fragment length polymorphism（RFLP） analysis of XbaⅠand PvuⅡpolymorphisms of ERα gene was performed to determine the genotype distribution of ERα gene.RELF was represented by PP,Pp,pp（PvuⅡ） and XX,Xx,xx（XbaⅠ）.Results X genotypic frequency was 35.0% and 24.5% in case group and control group,respectively（OR=1.659,P=0.022）.P genotypic frequency was 23.0% and 34.5% in case group and control group,respectively（OR=0.567,P=0.011）.RFLP of ERα gene presented polymorphic distrbution in both groups.Conclusion ERα gene polymorphism is associated with endometrial hyperplasia.X allele may be a risk factor and P allele may be a protective factor in endometrial hyperplasia.