肠腹宁颗粒及其主要成分总皂苷体外抗菌实验研究

The Vitro Antibacterial Study of Changfuning Granules and it's Main Components Total Saponins

目的:探讨肠腹宁颗粒及其主要成分总皂苷的抑菌作用。方法:先提取肠腹宁颗粒中的总皂苷,然后通过纸片法,以抑菌圈大小比较肠腹宁颗粒不同剂量、总皂苷对大肠埃希菌、金黄色葡萄球菌、痢疾杆菌、伤寒杆菌等的抑菌强度。结果:不同剂量的肠腹宁颗粒及其主要成分总皂苷对大肠埃希菌、金黄色葡萄球菌、痢疾杆菌、伤寒杆菌均有抑制作用,而肠腹宁颗粒高剂量组及其主要成分总皂苷与空白对照组比较有统计学差异(P<0.05)。结论:实验证明肠腹宁颗粒及其中主要成分总皂苷有抑菌作用。同时实验也为肠腹宁颗粒治疗小儿腹泻、慢性结肠炎的独特疗效提供了依据,为新药研发打下了基础。

Objective：Make an approach to the bacteriostatic action of Changfuning Granules and it＇s main components total saponins.Methods：Firstly,extract the total saponins in Changfuning Granules.And then through paper strip method,base on the size of inhibition zone,compared to the bacteriostatic strength of different doses of Changfuning Granules and total saponins on Escherichia coli,Staphylococcus aureus,Bacillus dysenteriae and Salmonella typhi etc.Results：Different doses of Changfuning Granules and total saponins on Escherichia coli,Staphylococcus aureus,Bacillus dysenteriae and Salmonella typhi have inhibitory action.Compared with the control group,the high dose group of Changfuning Granules and it＇s main components total saponins has a significant difference（P0.05）.Conclusion：Changfuning Granules and it＇s main components total saponins has bacteriostatic action.It provides a scientific basis that Changfuning Granules has a unique effect for the treatment of children with diarrhea or chronic colitis.At the same time,lays foundation for new drug development.