明日叶查尔酮对荷瘤小鼠抗氧化能力影响作用的研究

Research of Ashitaba Chalcone on the Antioxidant Effect in Tumor-Bearing Mice

目的研究明日叶查尔酮(AC)对荷H22肝癌小鼠细胞抗氧化能力的影响。方法将50只荷H22肝癌小鼠随机分为肿瘤对照组、环磷酰胺组、明日叶查尔酮高、中、低剂量组共五组,每组10只。AC低、中、高剂量组每日分别灌胃给予8、16、32 mg.kg-1明日叶查尔酮,肿瘤对照组给予生理盐水,环磷酰胺(CTX)组隔日腹腔注射20 mg.kg-1的CTX。第11天处死小鼠,取瘤组织检测各组小鼠血清脂质过氧化物(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶水平(GSH-Px)、总抗氧化能力(T-AOC)等指标,用MTT法检测各组小鼠肿瘤细胞增殖活性。结果 AC各剂量组尤其是中、高剂量组能够显著提高小鼠细胞的抗氧化能力,与肿瘤对照组比较,差异均有统计学意义(P<0.01);肿瘤对照组肝癌细胞增殖活性为(1.135±0.032)U.mL-1,AC高剂量组为(0.716±0.018)U.mL-1,两者比较,差异有统计学意义(P<0.05)。结论明日叶查尔酮能增强荷H22肝癌小鼠细胞的抗氧化能力,抑制肿瘤细胞增殖活性。

Objective To study the impacts of ashitaba chalcone（AC）on cell antioxidant capacity in H22 hepatoma-bearing mice.Methods 50 H22 hepatoma-bearing mice were randomly divided into five groups：a tumor control group,a cytoxan（CTX）group,an AC high-dose group,an AC middle-dose group and an AC low-dose group,10 rats in each one.In AC low-dose,middle-dose and high-dose groups,AC of 8,16,32 mg/kg was administered by gastric infusion separately everyday.In tumor control group,physical saline was given.In CTX group,CTX was administered with intraperitoneal injection,20mg/kg once every two days.On the 11th day,all the mice were sacrificed and tumor tissue was collected for the determination of serum lipid peroxide（MDA）,superoxide（SOD）,glutathione peroxidase（GSH-Px）,total antioxidant capacity（T-AOC）levels,etc.MTT assay was adopted to determine the proliferation activity of tumor cell in mice of each group.Results Of AC groups,the antioxidant effect of cell in mice were significantly improved in AC middle-dose and AC high-dose groups,indicating significant statistical difference（P0.01）.The hepatoma cell proliferation activity was（1.135±0.032）U/mL in tumor control group and was（0.716±0.018）U/mL in AC high-dose group,presenting significant statistical difference in comparison（P0.05）.Conclusion Ashitaba chalcone can enhance the antioxidant capacity and inhibit the proliferative activity of tumor cell in H22 hepatoma-bearing mice.