HIV-1/2/O抗体联合诊断试剂盒的制备及其检测效果的研究

Prepare the diagnosis kits with combined antibody of HIV-1/2/O and evaluate its application

目的制备HIV-1/2/O抗体检测试剂盒并评估其检测效果。方法采用RT-PCR分别获取HIV-1型M、O组的gp41以及HIV-2gp36的优势表位基因,并通过柔性链将各表位基因嵌合,重组表达,亲和纯化、辣根过氧化酶标记,与包被抗原配对筛选,获取检测HIV抗体的双抗原试剂。结果成功嵌合各表位基因并获得表达,纯化后的抗原具有很高的抗原活性,检测灵敏度与国外试剂相当。结论采用柔性链技术嵌合表达制备的HIV-1/2/O抗体联合诊断试剂盒对HIVO组病毒株的检测具有较高灵敏度。

Objective To prepare HIV-1/2/O test kits and to evaluate their test results. Methods RT-PCR for HIV-1 type were M,O groups of the gp41 and HIV-2 gp36 epitope gene of the advantages,and through flexible chain to the epitope chimeric gene,recombinant expression,affinity purified,horseradish off oxidase marker, paired with the coating antigen screening for detec- tion of HIV antibodies in double antigen reagents. Results The chimeric gene and the epitope was expressed,purified antigen with high antigenicity, sensitivity and considerable foreign agent. Conclusion flexible chain technology chimeric antibodies prepare HIV1/2/O joint diagnostic kit for HIV O Group virus strains detected with high sensitivity.