几类低等生物的核纤层与核纤层的起源进化研究

NUCLEAN LANINA OF SEVERAL GROUPS OF LOWER UNICELLULAR EUKARYOTES AND THE EVOLUTION OF NUCLEAR LAMINA

综合分析了本实验室多年来在几类具重要进化地位的低等单细胞真核生物上所进行的核纤层结构与成分的研究结果，并结合发育生物学等方面的资料，认为核纤层结构在真核细胞起源进化的初期即已起源形成，它的起源形成是"原核"进化成"真核"的重要前提条件之一；核纤层蛋白（基因）的起源分化过程应该是：首先起源产生B型蛋白（基因），在此基础上分化出A型蛋白（基因），最后形成了现存高等真核细胞的核纤层蛋白（基因）家族。

Nuclear laminas of several groups of lower unicellular were studied by sequential extraction, an embed-ment-free EM, Western blot analysis. The results of EM showed that Eugenla had a typical nuclear lamina structure, Dinoflagellates also had this structure but in me cells it was rot so obvious as that in higher eukarytes , whilein the most primitive eukaryote Archezoa, the nuclear lamina was not a completed layer structure and was undistin-guished. Western blot analysis showed that both Eugenla and Dinoflagellates had a major 67ku and a minor 70 kulamina plypeptides (lamin), which wee similar to lamin B (one of B-type lamins) and lamin A (one of A-typelamins) of mammalia respectively. Archezoa only had a 67ku lamin similar to lamin B of mammalia. According tothese, in addition t0 data 0f expression of lamins during development, it was suggested that lamina originated veryearly in the progression from prokaryotic cell to eukaryotic cell, and in lamina family B-type gene originated first andthen A-type gene derived from there.