CD133、β-catenin在胰腺癌中的表达及其临床关联性

Expression of CD133 and β-catenin in pancreatic cancer and clinical relevance

目的探讨CD133、β-catenin在胰腺癌中的表达及其临床意义。方法采用免疫组织化学方法(SP法)检测35例手术切除胰腺癌组织、癌旁组织、正常胰腺组织及胰周淋巴组织中CD133及β-catenin的表达情况。结果 CD133、β-catenin在胰腺癌中的表达阳性率分别为74.3%、62.9%,显著高于癌旁组织及正常组织中的阳性率,CD133的表达与淋巴转移、病理分期有关(P<0.05)、β-catenin的表达与淋巴转移、病理分期、分化程度有关(P<0.05),两者在蛋白表达水平上有明显相关性(P<0.05),CD133阳性表达组术后生存期明显短于阴性组(P<0.05)。结论胰腺癌中CD133的高表达与TNM分期及淋巴结转移有关。CD133高表达与胰腺癌预后差有关。β-catenin可能通过调节CD133的表达来调节胰腺癌干细胞的某些功能。

Objective To investigate the expression and significance of CD 133 and β-catenin in panereatie cancer and their relationship with the chnical pathologic characters. Methods Immunohistochemistry （SP） was used to detect the expression of CD133 and β-eatenin in 35 pancreatic cancer tissues ,the cancerous peripheral tissues, the normal pancreatic tissues and peripheral lymphonodes. Results The positive rates of CD133 and β-catenin expression in pancreatic carcinomas which were 74.3% and 62.9%, were significantly higher than that in adjacent normal tissue（P〈0.05）. The clinicopathological factors including pathologic staging and lymph node metastasis, were demonstrated significantly positive correlations with the expression of CD133 respectively（P〈0.05） The expression of β-catenin was associated with lymph node metastasis, pathological grade and histological differentiation,it also significantly correlated with the expression of CD 133 （P〈 0.05）. Consequently, the 5-year survival rate of CD133-positive patients was significantly lower than that of CD133-negative patients（P〈0.05）. Conclusions As important moleculars in prancreatic carcinoma stem cell, both CD133 and β--catenin play important roles in the tumorigenesis, development and metastasis of pancreatic carcinoma. β-eatenin may regulate certain functions of pancreatic cancer stem cell by regnlatting CD133＇s expression.