微透析技术结合色-质联用检测细胞外液神经递质研究进展

Measurement of Neurotransmitters from Extracellular Fluid in Brain by in Vivo Microdialysis and Chromatography-Mass Spectrometry

近30年,关于神经递质化学作用的大量信息被发现,同时也暴露出神经环路的复杂本性,多重神经递质系统间的交互作用以及不同受体对应神经化学物质的复杂效应。微透析已作为常规的测量活体组织系统的神经递质水平的实验技术,并在研究神经变性和精神性疾病机制以及鉴别治疗这些疾病的新药方面表现出特有的优势。而微透析取样技术与现代分析技术联用已成为生物学、医学、药学研究的强有力手段。最近,对体内收集的微透析液的分析,大多数聚焦在色-质联用技术的发展上,该技术能够给予更加灵敏和准确的神经递质的分析测量方法。现主要综述与精神神经疾病联系比较密切的几种细胞外液神经递质的检测方法。

During the last three decades,a great deal of information has been discovered about chemical neurotransmission.However,important processes,namely the complex nature of neuronal circuitry,the ＂cross talk＂ between multiple neurotransmitter systems,varying effects neurochemicals have at different receptors,are still being explored.Techniques such as microdialysis are routinely to measure neurotransmitter levels in living tissue systems.Moreover,microdialysis studies have proven to be valuable in the investigation neurodegenerative and psychiatric disease pathology,as well as in identifying novel drugs to treat such disorders.One particular performing these experiments is the requirement to couple microdialysis to sophisticated analytical equipment.Recently,considerable studies have been focused on the development of chromatographic–mass spectrometric techniques to provide more sensitive and accurate measurements of neurochemicals collected from in vivo microdialysis experiments.This review will provide a brief overview of the microdialysis as well as how microdialysis and chromatography–mass spectrometry are being used to measure extracellular levels of neurotransmitters.