棉团铁线莲总皂苷抗肿瘤作用研究

Antitumor Activities of Total Saponins from Clematis Hexapetala Pall.

目的：研究棉团铁线莲总皂苷的体内外抗肿瘤作用。方法：采用乙醇提取、大孔树脂除糖的方法制备棉团铁线莲总皂苷,以MTT法和移植性H22实体荷瘤小鼠为模型,检测不同剂量棉团铁线莲总皂苷的体内外抗肿瘤作用。结果：当质量浓度为0.1~1mg.mL-1时,其对体外培养的3株人肿瘤细胞HepG2、SMMC-7721和Hela的增值表现出显著的抑制作用,IC50依次为0.5298mg.mL-1、0.2047mg.mL-1、0.2877mg.mL-1。体内实验表明,棉团铁线莲总皂苷高（1.5g.kg-1）、中（0.75g.kg-1）、低（0.375g.kg-1）3剂量组也较好的抑制了荷瘤小鼠瘤块的生长,抑瘤率依次为41.55%、34.96%、32.37%;通过肿瘤组织的病理学切片观察,同样显示棉团铁线莲总皂苷具有较好的抗肿瘤作用。但比较给药后荷瘤小鼠的脾脏指数和胸腺指数后发现,它对荷瘤小鼠的免疫功能亦具有一定的抑制作用。结论：棉团铁线莲总皂苷具有较好的抗肿瘤作用,但对动物的免疫功能有所抑制。

Objective：To investigate the antitumor effect of total saponins of Clematis hexapetala Pall.Methods：The total saponins from Clematis hexapetala Pall.were prepared by ethanol extraction and macroporous resin.Its anti-tumor activity in vitro and in vivo was evaluated by MTT colorimetric assay and H22 cells-bearing mice models.Results：The results showed that the total saponins of Clematis hexapetala Pall.significantly inhibited proliferation of the human hepatoma cell lines HepG2,SMMC-7721 and the human cervical carcinoma cell Hela at the concentration of 0.1-1mg·mL-1.The IC50 value on these three tumor cells were 0.5298mg·mL-1,0.2047mg·mL-1 and 0.2877mg·mL-1 respectively.In vivo,high（1.5g·kg-1）,middle（0.75g·kg-1） and low（0.375g·kg-1）dose total saponins of Clematis hexapetala Pall.also significantly suppressed the tumor growth with tumor inhibition rate of 41.55%,34.96% and 32.37% respectively.The slice through the pathological observation of tumor tissue also indicated that it has good antitumor activity.However,it can also reduce the spleen index and displays certain inhibitory action to the immune organ of tumor-bearing mice.Conclusion：The total saponins from Clematis hexapetala Pall.has a significant antitumor effect,but it also can suppress immune function of experimentals animals in vivo.