葛根素对肝缺血-再灌注损伤兔血清IL-1β、TNF-α的影响

The Effect of Puerarin on Serum Interleukin-1β and Tumor Necrosis Factor-alpha of Rabbits with Hepatic Ischemia-reperfusion Injury

目的:观察葛根素(Pur)对家兔肝缺血-再灌注损伤(HIRI)兔血清白介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)水平及谷丙转氨酶(ALT)活性的影响,并探讨其肝脏保护机制。方法:30只家兔随机分为假手术对照组(C组)、缺血-再灌注组(I-R组)、尼莫地平组(Nim组)、葛根素I组(PurI组)和葛根素Ⅱ组(PurⅡ组),分别在缺血前、缺血45min、再灌注45min取静脉血,测定血清IL-1β、TNFα浓度及ALT活性,实验结束取肝组织观察肝细胞超微结构的变化。结果:肝缺血-再灌注期间,I-R组不同时点血清IL-1β、TNF-α水平及ALT活性明显高于C组,尤以再灌注45min为显著(P<0.05和P<0.01),肝细胞超微结构发生异常改变;PurI、PurⅡ及尼莫地平组不同时点血清IL-1β、TNF-α浓度及ALT活性显著低于I-R组(P<0.05和P<0.01),其超微结构异常改变较I-R组明显减轻。结论:葛根素能有效降低IL-1β及TNF-α的水平,这可能是它们减轻HIRI的又一重要机制。

Objective：To determine the inhibitory effect of puerarin on the level of serum interleukin-1β（IL-1β） and tumor necrosis factor-alpha（TNF-α） following hepatic ischemia and reperfusion injury（HIRI） in rabbits.Methods：30 rabbits which were randomly divided into five groups： control group（group C）,ischemia-reperfusion group（group I-R）,Nimodipine group（group Nim）,PurI group（group PurI） and PurⅡgroup（group PurⅡ）.Blood samples were obtained through vein to measure the serum concentration of IL-1βand TNF-α as well as alanine aminotransferase（ALT） activity in 20mins before ischemia,ischemia 45min and 45min after reperfusion.The ultrastructure of the hepatic tissue was observed.Results：The levels of IL-1βand TNF-α and the activity of ALT in serum at different time points in group I-R were higher significantly than that of the group C（P0.05 and P0.01）.The abnormal morphological changes of hepatocytes under electric microscope were more obvious in the group I-R than that in the group C.In the group PurI,PurⅡand Nim the levels of IL-1βand TNF-α and the activity of ALT in serum at different time points were remarkably lower than that of the group I-R（P0.05 and P0.01）,the abnormal morphyological changes of hepatocytes were all alleviated markedly after treatment of Pur and Nim.Conclusion：Puerarin could effectively decrease the levels of IL-1βand TNF-α and protect the liver from ischemia and reperfusion injury.