补髓生血颗粒对慢性再生障碍性贫血患者骨髓AKT磷酸化水平的影响

The Effect of Busuishengxue Granule on the Level of Phosphorylated AKT in CAA Patients' Bone Marrow

目的:观察补髓生血颗粒对CAA患者骨髓单个核细胞中AKT磷酸化水平变化,探讨补髓生血颗粒治疗CAA的作用机制。方法:将30例CAA患者予以补髓生血颗粒治疗6个月,另取28例健康志愿者作为正常对照组。采用蛋白质印迹方法(Western-blot)观察CAA患者治疗前后骨髓单个核细胞中pAKT-Ty308的表达水平。结果:CAA患者治疗前骨髓单个核细胞pAKT-Ty308的表达低于正常对照组(P<0.05),经补髓生血颗粒治疗后,pAKT-Ty308的表达有所上升,与治疗前比较有显著性差异(P<0.05)。结论:CAA患者骨髓单个核细胞PAKT-Ty308的水平均呈低水平表达,提示pAKT与CAA发病密切相关,补髓生血颗粒可能通过调节PAKT-Ty308的表达来改善CAA骨髓造血微环境。

Objective： To observe the effect of Busuishengxue Granule on the level of Phosphorylated AKT in CAA patients＇ bone marrow,and to explore the mechanism of Busuishengxue Granule on CAA.Methods： 30 CAA patients were treated with Busuishengxue Granule for 6 months,and another 10 healthy volunteers as normal control group.Use Western blot method（Western-blot） to observe the expression of PAKT-Ty308 in CAA patients＇ bone marrow mononuclear cells before and after treatment.Results： The expression of PAKT-Ty308 in CAA patients＇ bone marrow mononuclear cells were lower than that of normal control group（P0.05）,and the expression of PAKT-Ty308 was on the rise after treatment,so the comparison was significant difference before treatment.Conclusion： The expression of PAKT-Ty308 in CAA patients＇ bone marrow takes on low levels,so it suggests that PAKT is closely related to the pathogenesis of CAA,and Busuishengxue Granule may regulate the expression of PAKT-Ty308 to improve the CAA bone marrow microenvironment.