骨肉瘤组织中COX-2、VEGF的表达及意义

Expression and significance of COX-2 and VEGF in osteosarcoma

目的观察骨肉瘤组织中环氧化酶2(COX-2)、血管内皮生长因子(VEGF)的表达情况,并探讨其意义。方法对骨肉瘤患者45例行截肢术或瘤段切除假体置换术。术中分别切取骨肉瘤组织及对应癌旁(肿瘤组织边缘外5.0 cm处)组织标本。采用免疫组化SP法检测骨肉瘤组织及其癌旁组织中的COX-2、VEGF;以CD34为标记,计算微血管密度(MVD)。结果 COX-2和VEGF阳性表达率及MVD在骨肉瘤组织中分别为80.0%、75.6%和(43.6±11.4)条/HP,均高于癌旁组织的22.2%、17.8%和(13.5±7.3)条/HP,且三者与骨肉瘤的外科分期有关(P均<0.05)。COX-2与VEGF的表达呈正相关,COX-2和VEGF的表达均与MVD呈正相关(r分别为0.75、0.58和0.75,P均<0.05)。COX-2和VEGF同时阳性表达时,MVD显著增高(P<0.05)。结论 COX-2和VEGF在骨肉瘤组织中呈高表达,且与骨肉瘤外科分期呈正相关。二者共同参与了骨肉瘤的发生、发展,其机制可能是促进骨肉瘤组织中血管生成。

Objective To investigate the expression of cyclooxygenase 2（COX-2） and vascular endothelial growth factor（VEGF） in osteosarcoma and explore the significance.Methods 45 patients with osteosarcoma were treated with amputation or prosthesis replacement.Osteosarcoma and corresponding adjacent（5.0 cm at the outer edge of the tumor tissue） tissue samples were obtained during operation.The expression of COX-2,VEGF and MVD marked by CD34 of osteosarcoma and their adjacent tissues were detected by immuno histochemistry.Results The positive rates of COX-2,VEGF and MVD in osteosarcoma tissues were 80.0%,75.6% and（43.6±11.4）stripe/HP,respectively,which were higher than those in adjacent tissues（P all 0.05）.In patients with osteosarcoma,the expression of COX-2,VEGF and MVD were associated with Enneking stage（P all 0.05）.There was a positive relationship between the expression of COX-2 and VEGF,the expression of COX-2 and VEGF were positively correlated with MVD（r=0.75,0.58,respectively,P all 0.05）.The MVD values in both COX-2 and VEGF positive samples were higher than those in COX-2 negative and VEGF negative samples（P0.05）.Conclusions COX-2 and VEGF are highly expressed in osteosarcoma and expression of them are associated with surgical stage of osteosarcoma,may involved in the occurrence and development of osteosarcoma,the mechanism maybe contributing to angiogenesis in osteosarcoma.