肝癌患者血清、癌组织中IL-6表达及其性别差异

Expression and gender disparity of interleukin-6 in serum and tumor tissue in patients with hepatocellular carcinoma

目的观察肝细胞癌(肝癌)患者血清及癌组织中IL-6的表达及其性别差异。方法采用ELISA法检测不同性别肝癌患者术前及健康者外周血血清中的IL-6,免疫组化法检测肝癌组织中的IL-6。结果肝癌患者术前血清IL-6中位水平为4.82 pg/ml,健康者为2.31 pg/ml,两者比较,P<0.01;男性肝癌患者血清IL-6水平为4.96pg/ml,女性为3.87 pg/ml,两者比较,P<0.05。血清IL-6水平以3.51 pg/ml为cut-off值,诊断肝癌的敏感度与特异度分别为88.9%、87.5%;血清IL-6水平≥3.51 pg/ml者多为男性、肿瘤直径较大、血管内癌栓、肿瘤无包膜患者(P均<0.05)。肝癌组织中IL-6阳性率为77%(69/90),其中男性患者为84%(56/67),女性患者为57%(13/23),两者比较,P<0.05。结论肝癌患者血清IL-6水平比正常健康者明显升高,男性肝癌患者血清及癌组织中IL-6的表达均高于女性患者。

Objective To observe the expression of interleukin-6（IL-6） in serum and tumor tissue in hepatocellular carcinoma（HCC） patients and its gender disparity.Methods ELISA was used to detect the circulating serum level of IL-6 in different gender HCC patients before operation and in normal healthy.Immunohistochemical（IHC） was used to detect the expression of IL-6 in HCC tissue.Results The median serum levels of IL-6 in patients with HCC was 4.82 pg/ml,which in normal healthy people was 2.31 pg/ml.The median serum levels of IL-6 were significantly higher in patients with HCC than in healthy people,P0.01.Within HCC patients,the median level of IL-6 in male patients was 4.96 pg/ml and in female patients was 3.87 pg/ml,the median level of IL-6 were significantly higher in male patients than in female patients,P0.05.Under the ROC curve,3.51 pg/ml was set as the serum level of IL-6 cut-off value,and the sensitivity and specificity for detection of HCC were 88.9%,87.5%,respectively.Serum levels of IL-6 ≥ 3.51 pg/ml mainly focused on men,a larger tumor diameter,intravascular thrombosis,no capsule HCC patients.The IHC showed the positive rates of IL-6 in HCC were 77%（69/90）,and in male and female were 84%（56/67）,57%（13/23）,respectively.The positive rates of IL-6 were significantly higher in male HCC patients than in female HCC patients,P0.05.Conclusions The levels of serum IL-6 elevate significantly higher in HCC patients than in normal healthy people,and the level of IL-6 in serum and tumor tissue in male HCC patients are higher than in female patients.