结直肠癌患者淋巴结微转移与外周血NK细胞活性、IL-2、IL-12水平的关系

Correlation between the expression of CK20 mRNA in regional lymph nodes with serum level of NK,IL-2,IL-12 in colorectal cancer patients

目的探讨结直肠癌患者淋巴结微转移与外周血NK细胞活性及IL-2、IL-12水平的关系。方法采用流式细胞仪检测21例结直肠癌患者外周血NK细胞活性,ELLISA法检测患者外周血中的IL-2、IL-12;手术取肿瘤周围系膜淋巴结281枚,采用RT-PCR法检测淋巴结组织中的细胞角蛋白20(CK20)mRNA,以判断淋巴结微转移情况。结果 21例中有淋巴结微转移(CK20 mRNA阳性)者10例(140枚);无淋巴结微转移者外周血NK细胞活性及IL-2、IL-12水平高于有微转移者(P均<0.05);且淋巴结微转移与NK细胞活性及IL-2、IL-12水平呈负相关(r分别为-0.532、-0.834、-0.819,P均<0.05)。结论结直肠癌患者淋巴结微转移的发生与外周血NK细胞活性及IL-2、IL-12水平低下有关。

Objective To study the correlation between the expression of CK20 mRNA in regional lymph nodes with serum level of NK、IL-2、IL-12 in colorectal cancer patients.Methods CK20 mRNA in 281 lymph nodes of 21 colorectal cancer patientswere detected by RT-PCR;the serum level of NK cells were detected by flow cytometry and IL-2,IL-12,sIL-2R were detected by EILSA method.Results CK20 mRNA expression were positive in 140 lymph nodes of 10 patients（49.8%）.Before the operation,the serum level of NK cells,IL-2,IL-12 in the 11 patients whose CK20 mRNA in regional lymph nodes were negative expression were higher than the other 10 positive expression patients＇（P0.05）.The serum level of NK cells,IL-2,IL-12 were negative correlated with the positive rate of CK-20 mRNA（P0.05）.Conclusion The positive micrometastasis in lymph nodes are correlated significantly with low level of NK cells、IL-2、IL-12 in colorectal cancer patients.