山东肿足蕨总黄酮的提取工艺优化

Process optimization for extraction of total flavonoids from hypodematium sinense iwatuki

目的研究山东肿足蕨总黄酮的最佳提取方法。方法以山东肿足蕨总黄酮的含量为考察指标,通过单因素试验和正交试验的方法确定山东肿足蕨总黄酮的最佳提取方法。结果最佳提取工艺确定为用20倍量的70%乙醇,超声提取3次,每次60 min。结论优选的工艺稳定可行,可作为山东肿足蕨中总黄酮的提取工艺。

Objective To explore the extraction process of total flavones from Hypodematium sinense Iwatuki.Methods Using single factor experiment and orthogonal test the optimal extraction condition was determined from total flavones of Hypodematium sinense Iwatuki.Results The optimal condition of extraction process was as following： adding 20 folds of 70% aqueous ethanol and ultrasonic extracting for 60 min×3 times.Conclusion The extraction technics is feasible and can be used as extraction process of total flavones from Hypodematium sinense Iwatuki.