应用Fura-Ⅱ/AM检测大鼠多形核中性粒细胞内游离钙浓度

Using Fura Ⅱ/AM to Examine the Concentration of Free Calcium in PMN of Rat

应用ＦｕｒａⅡ技术，检测大鼠无菌性炎症时腹腔多形核中性粒细胞（ＰＭＮ）内游离钙浓度（［Ｃａ２＋］ｉ）及中药锦灯笼苦味素Ｂ对其影响。结果：在静息状态下，ＰＭＮ内［Ｃａ２＋］ｉ为（３１９４３±１８９０５）ｎｍｏｌ／Ｌ，酵母多糖激活可使［Ｃａ２＋］ｉ成倍增加，两者相比Ｐ＜００５。中药可抑制［Ｃａ２＋］ｉ增加。各组之间差异显著（Ｐ＜００５）。并探讨了ＦｕｒａⅡ检测ＰＭＮ内［Ｃａ２＋］ｉ的可行性及证明中药可抑制［Ｃａ２＋］ｉ。

Using Fura Ⅱ technique, examine the concentration of free calcium in rat celiac polymorphonuclear neutrophile granulocyte (PMN) under aseptic inflammation condition and the effect of bitter principles B of the Chinese lantern plant on it. Results: In resting state, the [Ca 2+ ] in PMN was 319.43+189.05nmol/L, the activation by zymosan could increase the [Ca 2+ ] remarkably, P<0.05. The Chinese medicine can inhibit the increase of [Ca 2+ ]. Differences between the groups were apparent, P<0.05. The feasibility of using Fura Ⅱ to examine [Ca 2+ ] in PMN was discussed as well and it proved that the Chinese Medicine could inhibit [Ca 2+ ].