HBV前S区的基因克隆及序列测定

Cloning and sequencing of HBV PreS gene

目的：获取 Ｈ Ｂ Ｖ 前 Ｓ区基因（ Ｈ Ｂ Ｖ Ｐｒｅ Ｓ），并进行序列测定，为今后研究其机理及应用创造条件． 方法：用 Ｐ Ｃ Ｒ方法从含 Ｈ Ｂ Ｖ 基因组的模板中扩增 Ｐｒｅ Ｓ基因，重组入 Ｂｌｕｅｓｃｒｉｐｔ 载体，用自动测序仪，采用荧光素标记的引物，测定目的基因的序列． 结果：成功地扩增到 Ｐｒｅ Ｓ区全长基因，测出的序列与已知的 Ｈ Ｂ Ｖ 病毒的ａｄｒ 亚型的前 Ｓ区基因序列一致． 结论：构建了含 Ｐｒｅ Ｓ区基因的重组克隆，为以后的深入研究奠定了基础．

AIM:To get HBV PreS gene and to explore its action,mechanism and application. METHEDS: HBV PreS gene was amplified by PCR from the template containing HBV genomic DNA and cloned into plasmid Bluescript. The nucleotide sequence of the target DNA was determined using Perkin Elmer Amplied Biosystems 373 Sequencer with the forward and reverse primers. RESULTS: The nucleotide sequence was consistent with that of HBV adr subtype PreS gene. CONCLUSION: Recombinant HBV PreS gene clone is established for further research.