间隙连接基因cx32,cx43在肝细胞癌组织中的表达意义

Expression of gap junction genes connexin 32 and 43 in human hepatocellular carcinoma

目的： 探讨间隙连接基因 ｃｘ３２，ｃｘ４３ 与肝细胞癌（ Ｈ Ｃ Ｃ） 发生的关系和作用机制． 方法： 应用 Ｓ Ｐ 免疫组织化学法和原位杂交技术，研究 Ｈ Ｃ Ｃ ６１ 例和正常肝组织１４ 例中ｃｘ３２，ｃｘ４３ 基因的表达规律． 结果： Ｃｘ３２ 蛋白在 Ｈ Ｃ Ｃ Ⅰ，Ⅱ，Ⅲ 级和正常肝中的阳性率分别为 ５５．６ ％ ， ４２．１％ ，１８．２％和 ９２．９％ ； Ｃｘ４３ 蛋白的阳性率分别为 ４４．４％ ， ２６． ３％ ，１２１％ 和 ７８．６％ ． Ｃｘ３２， Ｃｘ４３ 在 Ｈ Ｃ Ｃ各级和正常肝组织中存在显著性差异 （ Ｐ＜ ０．０５）． 原位杂交结果显示， Ｈ Ｃ Ｃ和正常肝中 ｃｘ３２，ｃｘ４３ ｍ Ｒ Ｎ Ａ 均保持较高水平，ｃｘ３２ ｍ Ｒ Ｎ Ａ 在 Ｈ Ｃ Ｃ Ⅰ，Ⅱ，Ⅲ 级和正常肝组织中的阳性率分别为 ８８．９％ ，８４．２％ ，８７．９％ 和 ９２９％ ；ｃｘ４３ ｍ Ｒ Ｎ Ａ 为 ７７．８％ ，７８．６％ ，７８８％ 和８５．７％ ， ｃｘ３２ 与ｃｘ４３ 基因在 Ｈ Ｃ Ｃ各级和正常肝中无显著性差异． 结论： ｃｘ３２，ｃｘ４３ 基因在转录后、翻译水平的调控异常，是其蛋白表达降低，导致 Ｈ Ｃ Ｃ发生的重要分子机制．

AIM: To investigate the significance and mechanism of cx 32 and cx 43 mRNA and their proteins in hepatocarcinogenesis. METHODS: 61 cases of HCC and 14 cases of normal liver tissue were detected with immunohistochemical method and in situ hybridization (ISH). RESULTS: In HCC (Grade Ⅰ, Ⅱ and Ⅲ) and normal liver tissue, the detection rates of Cx32 protein were 55.6%, 42.1%, 18.2% and 92 9% respectively and those of Cx43 protein were 44.4%, 26 3%, 12.1% and 78.6% respectively. The detection rates of Cx32 and Cx43 proteins were significantly different in HCC and normal liver tissue ( P <0.05). ISH showed the positive rates of cx 32 genes in HCC (Grade Ⅰ, Ⅱ and Ⅲ ) and normal liver tissue were 88.9%, 84.2%, 87.9% and 92.9% respectively,and those of cx 43 gene were 77.8%, 78 6%, 78.8% and 85.7% respectively. The positive rates of cx 32 gene and cx 43 gene had no significant difference in HCC and normal liver tissue. CONCLUSION: The aberrant location of Cx32 and Cx43 proteins are in some way responsible for hepatocarcinogenesis, which may result from the defect of cx gene in post translational process.