p16与PCNA表达与脑胶质瘤分级的相关性及意义

Correlation between the expression of p16 and PCNA in grades of glioma

目的： 研究ｐ１６ 与增殖性细胞核抗原（ Ｐ Ｃ Ｎ Ａ）蛋白在各级人脑胶质瘤中的表达率及与胶质瘤病理分级的关系．方法： 利用免疫组化方法检测５２ 例胶质瘤手术标本中ｐ１６ 与 Ｐ Ｃ Ｎ Ａ 蛋白的表达水平，并比较二者的相关性，及其与胶质瘤病理分级的关系． 结果： 随着胶质瘤病理级别的升高ｐ１６ 蛋白的表达率逐渐降低， Ⅰ～Ⅳ级阳性率分别为 ７５． ０％ ，６４７％ ，４２．８％ 和３３．３％ ，低度恶性组（Ⅰ～Ⅱ级）与高度恶性组（Ⅲ～Ⅳ级）有显著差异（ Ｐ＜ ０．０５）． Ｐ Ｃ Ｎ Ａ 表达强度随胶质瘤病理级别的升高而增加（ Ｐ＜ ０．０５）． 所选病例的平均 Ｐ Ｃ Ｎ Ａ 指数（ Ｐ Ｃ Ｎ Ａ Ｌ Ｉ）为 ２７．１， ｐ１６ 与 Ｐ Ｃ Ｎ Ａ 表达存在负相关（ｒ＝ ０．４８５， Ｐ＜ ０．０５）． 结论： ｐ１６ 及 Ｐ Ｃ Ｎ Ａ 蛋白的表达对分析判断胶质瘤病理分级的适用性及恶性转化具有一定意义．

AIM: To investigate the expression of p16 and PCNA protein in glioma and their correlation with pathological characteristics of glioma. METHODS: The expression of p16 and PCNA protein expression were observed by immunohistochemical method in 52 formalin fixed paraffin embedded glioma sections. RESULTS: Expressions of p16 protein significantly decreased with the increase of grades of glioma malignancy. The levels of p16 deletion between low malignant group(Ⅰ~Ⅱ)and high malignant group(Ⅲ~Ⅳ) were significantly different( P <0.05). Expressions of PCNA protein increased with the pathological grades of glioma( P <0.05). The mean PCNA label index (PCNA LI) was 27.1 and glioma with low PCNA LI had higher rates of p16 protein expression( P <0.05). CONCLUSION: Expressions of p16 and PCNA are of some significance in evaluating the pathological grades and biological behavior of glioma.