BALB/C近交系小鼠腭裂模型的建立及形态学观察

The establishment of cleft palate model and observation of its morphogenesis in BALB/C mice

目的：研究维甲酸和地塞米松联合用药对ＢＡＬＢ／Ｃ近交系小鼠腭部发育的影响。方法：将ＢＡＬＢ／Ｃ系孕鼠分为给药组和对照组，在妊娠第１２ｄ分别给药，并于妊娠期第１３８，１３２０，１４８，１４２０，１５８，１５２０，１６８ｄ处死孕鼠，取胎鼠头部做冠状切片，ＨＥ染色，进行观察。结果：维甲酸和地塞米松联合用药可诱导ＢＡＬＢ／Ｃ系小鼠形成腭裂，其主要原因是腭突上抬延迟。结论：维甲酸和地塞米松联合应用诱导ＢＡＬＢ／Ｃ近交系小鼠形成腭裂是一种稳定的腭裂动物模型。

Objective: To study the effects of retinoic acid(RA) and dexamethasone (DEX) on the palate development in BALB/C mice. Method: Pregnant BALB/C mice were divided into treated group and control group.In the treated group,the mice were dosed on GD12 with RA(100 mg/kg) and DEX(10 mg/kg).The mice were killed on day 138,1220,148,1420,158,1520,168,respectively.The coronal sections of the heads of the embryonic mice were prepared,stained with HE and observed microscopically. Result: Cleft palate was induced in BALB/C mice by the coadministration of RA with DEX,because the development of palate shelves was delayed. Conclusion: Coadministion of RA with DEX can induce cleft palate in BALB/C mice,and can be used to establish stable model of cleft palate.