口腔粘膜癌变过程中细胞角蛋白CK10和CK13的变化

Expressionof CK10 and CK13 in oral carcinogenesis

目的：探讨细胞角蛋白ＣＫ１０和ＣＫ１３在口腔粘膜癌变过程的各阶段组织中的分布和表达强度。方法：用ＬＳＡＢ免疫组化染色方法对口腔正常粘膜、上皮单纯增生、上皮异常增生和口腔磷癌切片进行染色，光镜观察。结果：正常颊粘膜和口底舌腹粘膜基底上层ＣＫ１０表达阴性，ＣＫ１３表达强阳性；上皮过度不全角化时，ＣＫ１０表达可为阳性，ＣＫ１３表达减少；上皮过度正角化时，ＣＫ１０在基底上层呈强阳性表达，ＣＫ１３表达进一步减少甚至缺失。随上皮异常增生程度加重，基底上层细胞ＣＫ１０和ＣＫ１３表达减少甚至缺失。在口腔浸润癌中，不管分化程度如何，ＣＫ１３均表达阴性，而ＣＫ１０在分化较好的癌细胞及癌巢中呈阳性表达。结论：ＣＫ１３表达减少或缺失可作为口腔癌前病变诊断的辅助指标，ＣＫ１０可为口腔癌的分化程度及其预后判定提供帮助。

Objective: To investigate the distribution and expression of Cytokeratin 10(CK10) and cytokeratin 13(CK13) in oral carcinogenesis. Methods: LSAB immunohistochemical method was used to stain the tissue sections of normal oral mucosa,epithelial hyperplasia, epithelial dysplasia and oral squamous cell carcinomas.The stained sections were observed under light microscopy.The results were described and analysed semiquantitatively with rank sum test. Results:CK10 was negative and CK13 was strongly positive in normal buccal mucosa,ventral tongue and mouth floor.CK10 was positive and CK13 expression decreased in parahyperkeratosis.CK10 was strongly expressed and CK13 decreased or disappeared in orthohyperkeratosis.With the development of dysplasia CK10 and CK13 expression decreased,even disappeared.CK13 was negative in oral invasive cancer cells,but CK10 was positive in cancer nest or some well differentiated cancer cells. Conclusion:WT5BZThe reduction or disappearance of CK13 expression can be one of the a