中心组合设计优化东方拟无枝酸菌发酵生产万古霉素的微量金属离子

Optimization of Trace Metal Ions for Vancomycin-Production Fermented from Amycolatopsis Orientalis V-0704 by Central Composite Design

对万古霉素生产菌东方拟无枝酸菌(Amycolatopsis orientalis)V-0704摇瓶培养48 h时发酵培养基中添加的几种微量金属离子进行了优化。依次通过部分因子析因设计、最速上升实验、中心组合设计,并利用统计学软件Minitab对实验数据进行分析,得到一组较优的微量金属离子组合:Co2+浓度2.486×10-5g.mL-1、Mo6+浓度2.486×10-5g.mL-1,添加这些金属离子摇瓶培养72 h的万古霉素平均效价达到4946 U.mL-1,较未加金属离子提高了17%。

Adding trace metal ions while the production of vancomycin fermented from Amycolatopsis orientalis V-0704 was optimized.Two level design for the trace metal ions showed that Co2＋ and Mo6＋ were the main influential factors for the production of vancomycin.The fractional factorial design,steepest ascent design and central composite design were employed orderly to optimize the chemical defined medium for the production of vancomycin by Minitab software.Finally the composition of the trace metal ions was obtained as follows： Co2＋ concentration of 2.486×10-5g·mL-1,Mo6＋ concentration of 2.486×10-5g·mL-1.The vancomycin yield adding the optimized trace metal ions was 4946 U·mL-1,which was increased by 17% compared with non-adding metal ions.