摘要
目的了解冷冻同种异体肌腱移植术后宿主的免疫排斥反应。方法采用BALB/C小鼠肌袋模型,植入冷冻C57小鼠跟腱,观察术后各期宿主血清微量细胞毒性、局部组织浆IL-2水平和局部组织学变化。结果(1)经冷冻/冻干处理组淋巴细胞毒性、IL-2活性显著低于未处理组(P<0.01),但仍高于自体组(P<0.01)。(2)处理各组间细胞毒性无差异(P>0.05),而IL-2活性-196℃组高于其他组(P<0.01)。(3)各组局部组织淋巴细胞浸润程度与细胞毒反应相符。结论经冷冻/冻干处理后,同种异体肌腱移植免疫排斥反应显著降低,但未消失。
Objective In order to investigate the immune rejection response of host to the implanted achilles tendon allografts of C 57 mice in BALB/C mice model. Methods The dryly frozen, deeply frozen (-196℃) and frozen (-80℃) achilles tendons of C 57 mice were respectively treated as Group A,B,C while fresh tendons allografts served as positive control (Group D) and autografts as negative control (Group E). Immunologic and histologic characters were observed at 2, 7, 14, 28, 42 days, separately. Results (1) The contents of lymphocytotoxicity and IL-2 showed markedly lower in Group A,B,C than in Group D ( P < 0.01 ), but were still higher than in Group E. (2) The IL-2 level was significant difference ( P < 0.01 ) between Group B and A or C. (3) The histologic immuneresponse in Group A,B,C abated more greatly than in Group D, but the local tissue was still infiltrated by monocytes and lymphocytes. Conclusions The immune rejection response is abated markedly but does not disappear in the treated achilles tendons of allografts.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
1999年第4期275-277,共3页
Chinese Journal of Trauma
关键词
移植免疫学
细胞毒性
肌腱移植
Transplantation, homologous Transplantation Immunology Tendon Cytotoxicity, immunologic Interleukin-2 Histology