人白细胞干扰素对子宫内膜腺癌细胞系的作用研究

Effect of Interferon Alpha on Endometrial Adenocarcinoma Cell Line

目的观察人白细胞干扰素（ＩＦＮα）对我们建立的子宫内膜腺癌细胞系（ＪＥＣ）的细胞生长和细胞周期的影响。方法将不同浓度：２０００ｕ／ｍｌ、５００ｕ／ｍｌ、１００ｕ／ｍｌ的ＩＦＮα与ＪＥＣ共同培养７天，检测ＪＥＣ细胞生长曲线、细胞的凝集素受体表达，并用流式细胞仪分析细胞周期的变化。结果和结论ＩＦＮα对ＪＥＣ细胞的增殖有抑制作用，浓度越大，抑制作用越明显，但不影响凝集素受体的表达；ＩＦＮα可使ＪＥＣ细胞Ｇ１／Ｇ０期增加、Ｓ期增加和Ｇ２／Ｍ期阻滞，前者与ＩＦＮα浓度呈正相关，而后者则为负相关。

Objective To observe effect of interferon alpha on cell growth and cell cycles of human endometrial adenocarcinoma cell line (JEC) established in our laboratory. Methods Effect of cell growth curve and lectin receptors on the cells were detected after incubation with different concentration at 2000 u/ml,500 u/ml,100 u/ml of interferon alpha for 7 days,and alteration of cell cycle were analysed using flow cytometry.Results and Conclusion Interferon alpha can inhibit the proliferation of JEC and the growth of the cells were dose dependently suppressed.There were no changes in expression of lectin receptors.The cells showed G 1/G 2 and S increse,but G 2/M arrest,it was positive correlation between the former and the concentration of interferon alpha,however,the two latters were negative correlation.