摘要
目的研究视黄酸对胃癌细胞的诱导分化及其相关酶活性的影响。方法１０－６ｍｏｌ／Ｌ的全反式视黄酸（ＡＴＲＡ）处理胃癌细胞株ＢＧＣ８２３和ＭＫＮ４５，用流式细胞术检测细胞周期的分布，以酶标仪测定相关分化标志酶的活性，用３ＨＴｄＲ掺入法测定ＤＮＡ合成速度和ＭＴＴ法检测细胞的生长。结果ＡＴＲＡ使ＢＧＣ８２３细胞出现Ｇ０／Ｇ１期停滞，分化标志酶ＬＤＨ、ＡＬＰ和βＧ的活性下降，抑制率分别为２２４％，３２９４％和４１３５％，使ＤＮＡ合成速率下降４１４３％，生长抑制率为６１０３％；但不能改变ＭＫＮ４５细胞的上述指标。结论ＡＴＲＡ能诱导ＢＧＣ８２３细胞分化并抑制其恶性生长，对ＭＫＮ４５细胞则没有这种作用。
Objective To explore effects of retinoic acid on gastric cancer cells' differentiation and relevant enzmye activity.Methods Treated with 10 -6 mol/L all trans retinoic acid(ATRA),gastric cancer cells,BGC 823 and MKN 45,were measured by flow cytometry for cell cycle distribution,by microplate reader for enzyme activity,by 3H TdR incorporation method for DNA synthetic speed and by MTT method for cell growth rate.Results After treated with ATRA,BGC 823 was lead to stay in G0/G1 phase,while LDH,ALP and β G activity was suppressed,the inhibition rates were 22 40%,32 94% and 41 35%,respectively.DNA synthetic speed was decreased by 41 43% and growth inhibition rate was 61 03%.Nevertheless,MKN 45 can't be changed as BGC 823.Conclusion BGC 823,but not MKN 45,can be induced differentiation and cell growth can be suppressed by ATRA.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
1999年第4期252-254,共3页
Cancer Research on Prevention and Treatment
基金
福建省自然科学基金
关键词
视黄酸
胃癌细胞
诱导分化
细胞周期
酶活性
Retinoic acid
Gastric cancer cells
Differentiation
Cell cycle
Enzyme activity
